Dissolve, warming somewhat. Sterilize within an autoclave using a validated cycle, in a temperature not exceeding 115
Retail store the organized culture media in air limited flask managed room temperature at controlled atmosphere
Transfer the bacterial cultures, media’s and streaking loops In the sub culturing place below Biosafety cupboard.
In GPT failure situations, again put together contemporary number of dehydrated media from the exact media container and carry out GPT once more.
For broth lifestyle media lavish growth of microorganism ought to be observed similar to the Formerly acquired with Earlier tested and accredited batch of medium happens.
– Pertumbuhan Staphylococcus aureus pada media bets baru harus sebanding dengan media bets sebelumnya yang telah lulus uji GPT
Following a specified incubation time period, notice the plates for growth and count the colonies received in Every single plate and document the results in Annexure-II.
Hi. For GPT on contact plates, should really I exploit A further Get hold of plate batch since the reference or is it Alright to implement a 90mm plate for comparison?
Choose the lifestyle with sterilized loop and transfer it in to the respective media aseptically as described down below in table1.
GPT of that society media complies While using the test for St. aureus if no yellow/white colonies
– Hasil pembacaan kemudian disesuaikan dengan kriteria keberterimaan sepeerti contoh berikut ini:
“Your business mentioned to our investigator that you do not carry out growth promotion on Every single lot of ready media to make certain your plates are suited to use in microbial testing of incoming factors, concluded drug products, and your water method.”
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